reversed phase hplc造句
例句與造句
- the method for the determination of amino acid neurotransmitters in brain by reverse phase hplc system with the precolumn phthali aldehyde derivatization
柱前衍生反相高效液相色譜法測定腦組織中氨基酸類神經(jīng)遞質(zhì) - it is introduced that the quantitative analysis of trichlorfon by reverse phase hplc, using acetonitrile and water ( 50 : 50 ) as mobile phase, using cn column and ultraviolet detector
摘要本文采用即以乙腈+水(50+50)為流動相,用cn柱和紫外檢測器分離測定敵百蟲原藥及40%敵?毒乳油中敵百蟲反相高效液相色譜法。 - the quantitative analysis of 25 % chlorpyrifos and triazophos ec by reverse phase hplc was introduced, using acetonitrile and water ( 74 : 26 ) as mobile phase, using c18 column and ultraviolt detector
摘要本文敘述了采用反相高效液相色譜法即以乙腈+水為流動相,c18柱和紫外檢測器同柱分離側(cè)定25%毒?唑乳油中的毒死蜱和三唑磷。 - imidacloprid and triazophos in mixture were determined simultareously by reverse phase hplc, methanol and acetonitrile and water and glacial acetic acid as mobile phase, c18 column and uv200 detecter
摘要采用c18不銹鋼柱和uv200型檢測器,以甲醇?乙腈?水的混合液為流動相,用反相高效液相色譜法對吡蟲啉?三唑磷復(fù)配制劑進(jìn)行定量分析測定。 - the crude venom contains a mixture of compounds with different types of biological activities . two novel peptide neueotoxins were purified from the venom of chilobrachys jingzhao by using combination of ion-exchange and reverse phase hplc
海南敬釗纓毛蛛(chilobrachysjingzhao)毒素是最近在海南發(fā)現(xiàn)的蜘蛛新種,該蜘蛛粗毒中含有多種活性多肽和蛋白質(zhì)成分。 - It's difficult to find reversed phase hplc in a sentence. 用reversed phase hplc造句挺難的
- secondly, determination of the antimicrobial activities of synthetic hpab-. i ) the mature peptide of hpab was synthesized by fomc method and purified by reverse phase hplc . the molecular weight of synthesis peptide was 4334.96da characterized by mass spectrometry, highly agreed with the theoretical mw of hpab-3
2.hpab一p抗菌活性初步鑒定:采用固相法合成了hpab一p成熟膚,經(jīng)即一hplc純化后用質(zhì)譜測定合成膚的分子量為4334.%da,與其理論分子量相符。 - both hainantoxin-iv ( hntx-iv ) and . hainantoxin-v ( hntx-v ) w ere peptide toxins isolated from the venom of the spider seleconosmia hainana by means of ion-exchange chromatograph and reverse phase hplc . hntx-iv, 3989 da, contained 35 amino acid residues with three disulfide bonds, while the molecular weight of hntx-v was 3972 da and had three disulfide bonds, too
海南捕鳥蛛毒素-(hntx-)和海南捕鳥蛛毒素-(hntx-)是從海南捕鳥蛛粗毒中經(jīng)陽離子交換和反相hplc柱層析分離出來的兩種神經(jīng)毒素,maldi-tof質(zhì)譜鑒定前者相對分子質(zhì)量為3,989da,含有三對二硫鍵,由35個氨基酸殘基組成;后者相對分子質(zhì)量為3,972da,與前者只相差16個質(zhì)子,也含有三對二硫鍵。 - the hwtx-i gene was chemically synthesized according to its known cdna sequence, the gene was inserted into vector ppic9k which contained aoxj promotor and the sequence of a secreting signal peptide-a-factor, the cloning ppic9k / hwtx-i was constructed and confirmed by two-step pcr and dna sequence analysis, then it was transformed into host strain gs115, a his + muts cell line was screened and multicopy transformants were screened by various g418 concentrations, the multicopy transformant was named gh1 . gh1 was cultivated in flasks . after 6 days of induction by 0.5 % methanol, the supernatant was checked by 16.5 % tricine-sds page, which showed there was a band in the position of 3.5-6.1kd, then it was isolated and desalted by ultrofiltration followed by ion exchange of cm column, after reverse phase hplc of ci8 and vacuum drying, the purified rhwtx-1 was obtained which was proved to be correct recombinant hwtx-i by tricine sds-page, maldi-tof mass spectrometry, amino acid composition analysis, the n-terminal amino acid sequence and its biological activity, the final field of the purified rhwtx-i was about 80mg / l, accounting for 23.6 % of it total secretory proteins
將帶有hwtx-基因的ppic9k經(jīng)blgii線性化后,轉(zhuǎn)化酵母宿主菌gs115原生質(zhì)體后經(jīng)篩選陽性克隆并經(jīng)表型鑒定為his~+mut~s酵母菌,進(jìn)一步用遺傳毒素g418篩選多拷貝的轉(zhuǎn)化菌株,命名為gh1;將gh1甲醇酵母菌用0.5的甲醇誘導(dǎo)表達(dá),發(fā)酵上清經(jīng)90飽和度的(nh_4)_2so_4沉淀,yw-3(mwc03000)的超濾膜超濾,再經(jīng)cm陽離子交換,c_(18)反相hplc純化得到分子量為4kd左右的組分,其中4289.05的組分經(jīng)質(zhì)譜鑒定,氨基酸組成分析和序列測定為正確的表達(dá)產(chǎn)物,生物學(xué)活性表明其活性為天然毒素活性70%,表達(dá)量為80mg/l。 - a neurotoxic peptide ( named huwentoxin-v ) was purified from the venom of the spider by a combination of ion exchange chromatography and reverse phase hplc . hwtx-v has 35 amino acid residues, with the molecular mass 4111.4da . the amino acid sequence has been determined as nh2-ecrwylggcsqdgdcckhlqchsn-yewcvwdgtfs-cooh, which consists of 6 cys, formed three pairs of disulfide bridges
本文報(bào)道從虎紋捕鳥蛛(selenocoimahuwena)粗毒中,結(jié)合陽離子交換和反相高效液相色譜分離純化出一種昆蟲毒素,命名為虎紋捕鳥蛛毒素-(huwentoxin-,hwtx-);其天然突變體(themutantofhuwentoxin,mhwtx-)也同時純化出來。
相鄰詞匯
- "reversed peristalsis"造句
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